731 PCR TECHNOLOGY

SC 857 731 :: PCR TECHNOLOGY FOR M.SC. STUDY

Course Overview

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Last Updated On 21 September 2025

6 Students Enrolled
16 Downloadable Resources

Course Content

Start Date: 18/06/24
Category: ภาควิชาชีวเคมี

General

Course organizer: Assoc. Prof. Dr. Khomsorn Lomthaisong
SC 857 731 course syllabus - 1st semester, 2025
Grading Standards

Topic 1 Introduction to PCR, Topic 2 PCR principle and type of PCR thermocyclers, Topic 3 Factors affecting PCR - Assoc. Prof. Dr. Gulsiri Senawong

Slide 731 PCR TECH - Topic 1-3
MID TERM SCORES - Part Aj-Gul

Topic 4 Advanced PCR techniques: Principles and Applications (4.1) Hot start PCR (4.2) Touchdown PCR (4.3) RT-PCR (4.4) Rapid amplification cDNA end (4.5) Nested PCR (4.6) LA-PCR (4.7) Asymmetric PCR (4.8) Inverse PCR - Assoc. Prof. Dr. Gulsiri Senawong

Slide 731 PCR TECH - Topic 4.1 - 4.8

(4.9) RAPD (4.10) AFLP (4.11) PCR-RFLP (4.12) SSR-PCR (4.13) ISSR-PCR (4.14) Multiplex PCR - Asst. Prof. Dr. Lapatrada Taemaitree

(4.15) Competitive PCR (4.16) Semi-quantitative RT-PCR & Real-time PCR - Assoc. Prof. Dr. Maysaya Thitisaksakul

Competitive PCR
Semi-quantitative RT-PCR & Real-time PCR

(4.17) Subtractive PCR (4.18) PCR differential display - Asst. Prof. Dr. Lapatrada Taemaitree

(4.19) Digital PCR (4.20) LAMP - Assoc. Prof. Dr. Gulsiri Senawong

Topic 5 Primer design (5.1) Gene sequence retrieval (5.2) Free programs for primer design (5.3) How to design primer - Assoc. Prof. Dr. Gulsiri Senawong

Slide specific & site-directed mutaganesis primers design
Slide degenerated primer design
Homework

Topic 6 Applications of PCR techniques (6.1) Sequencing (6.2) Genetic engineering and DNA mutagenesis - Asst. Prof. Dr. Chamaiporn Champasri

Applications

About the Instructor

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ชไมพร จำปาศรี

chamai

ชไมพร จำปาศรี

စာရင်းပေးသွင်းမှုဆိုင်ရာ ရွေးချယ်စရာများ

SC 857 731 :: PCR TECHNOLOGY FOR M.SC. STUDY

Skill Level: Beginner

ဧည့်သည် သုံးစွဲသူများ ဤသင်ရိုး/သင်တန်းအား အသုံးမပြုနိုင်ပါ။ ကျေးဇူးပြု၍ လော့ဂ်အင်ပြုလုပ်ပါ။

Price FREE

Enrol Now

Last Updated On 21 September 2025

6 Students Enrolled
16 Downloadable Resources

Course Features

Lectures9
Start Date18 Jun 2024
Skill level Beginner
Language English
Assessments Yes

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