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SC 857 731 :: PCR TECHNOLOGY FOR M.SC. STUDY
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General
Course organizer: Assoc. Prof. Dr. Khomsorn Lomthaisong
SC 857 731 course syllabus - 1st semester, 2025
Grading Standards
Topic 1 Introduction to PCR, Topic 2 PCR principle and type of PCR thermocyclers, Topic 3 Factors affecting PCR - Assoc. Prof. Dr. Gulsiri Senawong
Slide 731 PCR TECH - Topic 1-3
MID TERM SCORES - Part Aj-Gul
Topic 4 Advanced PCR techniques: Principles and Applications (4.1) Hot start PCR (4.2) Touchdown PCR (4.3) RT-PCR (4.4) Rapid amplification cDNA end (4.5) Nested PCR (4.6) LA-PCR (4.7) Asymmetric PCR (4.8) Inverse PCR - Assoc. Prof. Dr. Gulsiri Senawong
Slide 731 PCR TECH - Topic 4.1 - 4.8
(4.15) Competitive PCR (4.16) Semi-quantitative RT-PCR & Real-time PCR - Assoc. Prof. Dr. Maysaya Thitisaksakul
Competitive PCR
Semi-quantitative RT-PCR & Real-time PCR
(4.19) Digital PCR (4.20) LAMP - Assoc. Prof. Dr. Gulsiri Senawong
dPCR
Animation dPCR
LAMP
Animation strand displacement actvity
Animation LAMP
Topic 5 Primer design (5.1) Gene sequence retrieval (5.2) Free programs for primer design (5.3) How to design primer - Assoc. Prof. Dr. Gulsiri Senawong
Slide specific & site-directed mutaganesis primers design
Slide degenerated primer design
Homework
Topic 6 Applications of PCR techniques (6.1) Sequencing (6.2) Genetic engineering and DNA mutagenesis - Asst. Prof. Dr. Chamaiporn Champasri
Applications
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731 PCR TECHNOLOGY
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731 PCR TECHNOLOGY
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SC 857 731 :: PCR TECHNOLOGY FOR M.SC. STUDY
Teacher:
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Skill Level
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Beginner