Friday 10.00- noon Room MDBC1 Department of Biochemistry (Alternative room: 3505)
Course Co-ordinators:
1 Dec 2023 Regulation of gene expression in prokaryotes and eukaryotes: applications in medicine (WoL)
https://forms.gle/LVFfasGRQ3PrC56z5
8 Dec 2023 Pre-mRNA splicing and RNA editing (RnT)
22 Dec 2023 Gene silencing (siRNA and miRNA) (KS)
5 Jan 2024 Protein structure (PM)
Deadline is 8 Jan, 2024
12 Jan 2024 Protein splicing and protein editing (AS)
Deadline for submission: Mar 31, 2024
19 Jan 2024 Medical glycobiology (AS)
Assignments for Medical glyobiology: Deadline for submission Mar 31, 2024
Design experiments, using the provided information, to prove this sentence
“N-glycosylation is important for the function of tyrosinase enzyme”
Provided information:
The enzyme that is very important for N-glycosylation of the protein is oligosacharyl transferase (OST).
N-linked glycosylation inhibitor 1 (NGI-1) is recently found to be a potent OST inhibitor.
The activity of tyrosinase enzyme can be directly measured by DOPA oxidase assay.
Expression of tyrosinase in the cells and be determined by realtime-RT PCR and Western blotting.
The cells that highly express tyrosinase are MNT-1 and B16F10 melanoma cell lines.
N-linked glycan is mannose rich, which can be detected by concanavalin A lectin.
2 Feb 2024 Protein targeting (SL)
16 Feb 2024 Signal transduction and roles in medicine (CC)
23 Feb 2024 Oxidative stress and antioxidants (RnT)
1 Mar 2024 Special lecture: Dr. Arnatchai Maiuthed, Mahidol University
8 Mar 2024 Assigned reading (AR) (CC/KS)
Instruction:
Pre-class activities
1. Read article in title " Therapeutic strategies to target RAS-mutant cancers."
2. Discuss and prepare for group presentation. Students need to explain all figures with supporting information in the article.
In-class activities:
1. Group presentation [40 mins]
2. Question and answer [10 mins]
3. Post-test [10 mins]
Scores:
Total score for in-class activities is 10 % diving into two parts:
5% for group presentation and question and answer
5% for post-test