Topic outline

  • General

    MD637702 Medical Biochemistry and Molecular Biology (2 credits: 2-0-4)

    Second semester, Academic Year 2024


    Friday 10.00- noon Room MDBC1 Department of Biochemistry

    Course Co-ordinators:

    1. Assoc. Prof. Dr. Kanlayanee Sawanyawisuth (E-mail: kanlayanee@kkumail.com) 
    2. Asst. Prof. Dr. Chalongchai Chalermwat (E-mail: cchanl@kku.ac.th)

  • Topic 1

    29 Nov 2024 Regulation of gene expression in prokaryotes and eukaryotes: applications in medicine (WoL)

    • post-test URL

      https://forms.gle/LVFfasGRQ3PrC56z5

    • file 2.5MB PDF document Uploaded 28/11/24, 21:44

  • Topic 2

    13 Dec 2024 Pre-mRNA splicing and RNA editing (RnT)


  • Topic 3

    20 Dec 2024 Gene silencing (siRNA and miRNA) (KS)


  • Topic 4

    27 Dec 2024 Protein splicing and protein editing (OP)

    • Protein_Splicing 2-2567 File 5.5MB PDF document Uploaded 26/12/24, 23:08

      Assignment
      1.Read the given paper
      2.Select the most impressive application of protein splicing
      3.Briefly explain the application no more than 500 words
      4.Deadline submission is 16 Jan 2025
      5.send by email: orasapa@kku.ac.th 


    • Paper 1 File 1.2MB PDF document Uploaded 26/12/24, 22:43
    • Paper 2 File 2MB PDF document Uploaded 26/12/24, 22:43
    • Paper 3 File 6.4MB PDF document Uploaded 26/12/24, 22:44
    • Paper 4 File 1.7MB PDF document Uploaded 27/12/24, 09:43

  • Topic 5

    3 Jan 2025 Protein structure (PM)

  • Topic 6

    10 Jan 2025 Medical glycobiology (AS)

    • Med Glyco MD637702 File 10.7MB PDF document
    • Assignment Med Glyco File

      Assignments for Medical glyobiology: Date for discussion will be decided later

      Design experiments, using the provided information, to prove this sentence

      “N-glycosylation is important for the function of tyrosinase enzyme”

      Provided information:

      1. The enzyme that is very important for N-glycosylation of the protein is oligosacharyl transferase (OST).

      2. N-linked glycosylation inhibitor 1 (NGI-1) is recently found to be a potent OST inhibitor.

      3. The activity of tyrosinase enzyme can be directly measured by DOPA oxidase assay.

      4. Expression of tyrosinase in the cells and be determined by realtime-RT PCR and Western blotting.

      5. The cells that highly express tyrosinase are MNT-1 and B16F10 melanoma cell lines.

      6. N-linked glycan is mannose rich, which can be detected by concanavalin A lectin.


  • Exam 1

    17 Jan 2025 Exam 1 (Topic 1-6): KS/CC


    • Topic 7

      24 Jan 2025 Protein targeting (SL)



    • Topic 8

      31 Jan 2025 Enzyme catalysis (KK)

    • This topic

      Topic 9

      7 Feb 2025 Signal transduction and roles in medicine (CC)

    • Topic 10

      14 Feb 2025 Oxidative stress (RnT)


    • Topic 11: Special lecture

      21 Feb 2025 Assoc.Prof.Dr.Siriporn  Proungvitaya, Department of Medical Technology, Faculty of Associated Medical Sciences, KKU


    • Assigned Reading

      28 Feb 2025 Assigned reading (AR) (CC/KS) 

      Instruction: 

      Pre-class activities

      1. Read article in title " Therapeutic strategies to target RAS-mutant cancers." 

      2. Discuss and prepare for group presentation. Students need to explain all figures with supporting information in the article. 

      In-class activities:

      1. Group presentation [40 mins]

      2. Question and answer [10 mins]

      3. Post-test [10 mins]

      Scores: 

      Total score for in-class activities is 10 % diving into two parts: 

      5% for group presentation and question and answer  

      5% for post-test 

      Sincerely yours,
      CC/KS

       

    • Exam 2


      7 Mar 2025 Exam 2 (Topic 7-11)   KS/CC